Neoschizomer

Neoschizomers are restriction enzymes that recognize the same nucleotide sequence, but cleave at different sites. The first restriction enzyme discovered to recognize a sequence is called the prototype, and others that recognize the same sequence are isoschizomers. Neoschizomers are a subset of isoschizomers.^[1]

Recognition sequences and products of neoschizomers

For example, MaeII is the prototype enzyme for the sequence "ACGT", with the cleavage site A↓CGT. One of its neoschizomers, Tsp49I, also recognizes the sequence "ACGT", but cleaves at ACGT↓.^[2]

Another example is SmaI (CCC↓GGG), which is a neoschizomer of XmaI (C↓CCGGG).^[3]

Use in molecular biology

External links

• Rebase restriction enzyme database, http://rebase.neb.com/rebase/

See also

• Isoschizomer
• Isocaudomer
• List of restriction enzyme cutting sites