Phikmvvirus

Phikmvvirus is a genus of viruses that infect bacteria. There are currently 16 species in this genus including the type species Pseudomonas virus phiKMV.^[1][2] Bacteriophage phiKMV^[3] and its relatives are known to be highly virulent phages, producing large (3–15 mm (0.12–0.59 in) diameter) clear plaques on a susceptible host.^[4][5] The only reported exception is phage LKA1, which yields small plaques (1 mm (0.039 in)) surrounded by a halo. While all other P. aeruginosa-specific phikmvviruses use the Type IV pili as primary receptor, LKA1 particles attach to the bacterial lipopolysaccharide layer.

Phikmvvirus
Virus classification
(unranked):	Virus
Realm:	Duplodnaviria
Kingdom:	Heunggongvirae
Phylum:	Uroviricota
Class:	Caudoviricetes
Order:	Autographivirales
Subfamily:	Krylovirinae
Genus:	Phikmvvirus

Taxonomy

The following species are recognized:^[2]

• Pseudomonas virus 130-113
• Pseudomonas virus 15pyo
• Pseudomonas virus Ab05
• Pseudomonas virus ABTNL
• Pseudomonas virus DL62
• Pseudomonas virus kF77
• Pseudomonas virus LKD16
• Pseudomonas virus LUZ19
• Pseudomonas virus MPK6
• Pseudomonas virus MPK7
• Pseudomonas virus NFS
• Pseudomonas virus PAXYB1
• Pseudomonas virus phiKMV
• Pseudomonas virus PT2
• Pseudomonas virus PT5
• Pseudomonas virus RLP

Virology

Electron microscopic imaging of purified phage particles revealed these phages as typical members of the Podoviridae, with a head diameter of approximately 60 nm (2.4×10⁻⁶ in) and a stubby tail with a length of 8–10 nm. Although phiKMV phage resembles the well-studied podovirus T7 in overall genome architecture, it was the first known T7-like phage which encoded a single-subunit RNA polymerase gene downstream its DNA metabolism genes instead of in the early genomic region. Based on these properties, the genus Phikmvvirus is classified within the Autographiviridae.^[6]

Genus	Structure	Symmetry	Capsid	Genomic arrangement	Genomic segmentation
Phikmvvirus	Head-Tail	T=7	Non-enveloped	Linear	Monopartite

Life cycle

Viral replication is cytoplasmic. Entry into the host cell is achieved by absorption into the host cell. DNA-templated transcription is the method of transcription. The virus exits the host cell by lysis, and holin/endolysin/spanin proteins. Bacteria serve as the natural host. Transmission routes are passive diffusion.^[1]

Genus	Host details	Tissue tropism	Entry details	Release details	Replication site	Assembly site	Transmission
Phikmvvirus	Bacteria	None	Injection	Lysis	Cytoplasm	Cytoplasm	Passive diffusion