Protein N-terminal methyltransferase

Protein N-terminal methyltransferase (EC 2.1.1.244, NMT1 (gene), METTL11A (gene)) is an enzyme with systematic name S-adenosyl-L-methionine:N-terminal-(A,P,S)PK-(protein) methyltransferase.^[1][2] This enzyme catalyses the following chemical reaction

(1) 3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] ${\displaystyle \rightleftharpoons }$ 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein] (overall reaction)

(1a) S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-(A,S)PK-[protein]

(1b) S-adenosyl-L-methionine + N-terminal-N-methyl-N-(A,S)PK-[protein] ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-(A,S)PK-[protein]

(1c) S-adenosyl-L-methionine + N-terminal-N,N-dimethyl-N-(A,S)PK-serine-[protein] ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein]

(2) 2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] ${\displaystyle \rightleftharpoons }$ 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein] (overall reaction)

(2a) S-adenosyl-L-methionine + N-terminal-PPK-[protein] ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-PPK-[protein]

(2b) S-adenosyl-L-methionine + N-terminal-N-methyl-N-PPK-[protein] ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein]

Protein N-terminal methyltransferase
Identifiers
EC no.	2.1.1.244
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

This enzyme methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys.