Pseudomonas denitrificans

Pseudomonas denitrificans is a Gram-negative aerobic bacterium that performs denitrification. It was first isolated from garden soil in Vienna, Austria. It overproduces cobalamin (vitamin B₁₂), which it uses for methionine synthesis^[1] and it has been used for manufacture of the vitamin.^[2] Scientists at Rhône-Poulenc Rorer took a genetically engineered strain of the bacteria, in which eight of the cob genes involved in the biosynthesis of the vitamin had been overexpressed, to establish the complete sequence of methylation and other steps in the cobalamin pathway.^[3][4]

Pseudomonas denitrificans
Scientific classification
Domain:	Bacteria
Kingdom:	Pseudomonadati
Phylum:	Pseudomonadota
Class:	Gammaproteobacteria
Order:	Pseudomonadales
Family:	Pseudomonadaceae
Genus:	Pseudomonas
Species:	P. denitrificans
Binomial name
Pseudomonas denitrificans Bergey, et al. 1961
Type strain
ATCC 19244 CCEB 525 IAM 12023 NCIMB 1656 NCTC 1656 NRRL B-1028

Based on 16S rRNA analysis, P. denitrificans has been placed in the P. pertucinogena group.^[5]