RRNA small subunit pseudouridine methyltransferase Nep1

RRNA small subunit pseudouridine methyltransferase Nep1 (EC 2.1.1.260, Nep1, nucleolar essential protein 1) is an enzyme with systematic name S-adenosyl-L-methionine:18S rRNA (pseudouridine¹¹⁹¹-N¹)-methyltransferase.^[1][2][3] This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + pseudouridine¹¹⁹¹ in yeast 18S rRNA ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + N¹-methylpseudouridine¹¹⁹¹ in yeast 18S rRNA

RRNA small subunit pseudouridine methyltransferase Nep1
Identifiers
EC no.	2.1.1.260
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

This enzyme recognizes specific pseudouridine residues (Psi) in small subunits of ribosomal RNA based on the local RNA structure.

A point mutation in the ribosome biogenesis factor Nep1 impairs its nucleolar localisation and RNA binding and causes the Bowen-Conradi syndrome. ^[4] .^[5]