Scytalidopepsin B

Scytalidocarboxyl peptidase B, also known as Scytalidoglutamic peptidase and Scytalidopepsin B (EC 3.4.23.32, obsolete names include Scytalidium aspartic proteinase B, Ganoderma lucidum carboxyl proteinase, Ganoderma lucidum aspartic proteinase, Scytalidium lignicolum aspartic proteinase B, SLB) is a proteolytic enzyme.^{[1][2][3][4][5]} It was previously thought to be an aspartic protease, but determination of its molecular structure showed it to belong a novel group of proteases, glutamic protease.^[6][7]

Scytalidocarboxyl peptidase B
Structure of scytalidocarboxyl peptidase B, with cleaved peptide product in black and active site glutamate-glutamine dyad side chains in red. (PDB: 1S2K​)
Identifiers
EC no.	3.4.23.32
CAS no.	104781-89-7
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

The protease has a unique structure and a novel catalytic dyad (E136 and Q53) in its active site. The active-site residues, glutamic acid (E) and glutamine (Q), was used to coin the name of the family of proteases; eqolisins, to which Scytalidoglutamic peptidase B belongs.^[6]

This enzyme catalyses the following chemical reaction

Hydrolysis of proteins with broad specificity, cleaving Phe²⁴-Phe and Tyr²⁶–Thr but not Leu¹⁵-Tyr and Phe²⁵-Tyr in the B chain of insulin. It also cleaves the His⁶–Pro bond of angiotensin I, the ability to cleave a peptide bond with Pro in the P1′ position is unusual.

This endopeptidase is isolated from Scytalidium lignicolum. It is an acid protease, and is most active at pH 2.0 when casein is used as substrate. Eqolosins prefer bulky amino acid residues at the P1 site and small amino acid residues at the P1′ site.^[8] The substrate specificity of scytalidoglutamic peptidase is unique, particularly in the substrate preferences at the P3 (basic amino acid), P1′ (small amino acid) and P3′ (basic) positions.^[9]