km | Exit | Interchange | To | Remarks |
| | | North
FT 1 Saleng
FT 1 Kulai
FT 1 Simpang Renggam
FT 1 Ayer Hitam | |
FT 1 Skudai Highway Start/End of highway Start/End of dual carriageway |
27.1 | | Former Senai toll plaza location | | |
FT 1 27 | | Malaysian Highway Authority Southern Regional Office | Malaysian Highway Authority Southern Regional Office | North bound |
| 116 | Saleng Interchange |  Second Link Expressway Second Link Expressway (Senai Link)
Southwest
Second Link Expressway AH143 Tuas (Singapore)
Second Link Expressway AH143 Iskandar Puteri
North–South Expressway Southern Route AH2 Kuala Lumpur
Johor Premium Outlets | Off-ramp from Johor Bahru direction; on-ramp to northbound direction |
| | Senai Taman Senai Utama | | |
| 115 | Senai Senai Airport Highway Interchange | Northeast
FT 16 Senai Airport Highway
FT 16 Senai International Airport 
 Senai–Desaru Expressway Senai–Desaru Expressway
FT 3 AH18 Ulu Tiram
FT 3 AH18 Kota Tinggi
FT 17 Pasir Gudang
FT 90 Desaru
 Second Link Expressway Second Link Expressway
Second Link Expressway AH143 Tuas (Singapore)
Second Link Expressway AH143 Iskandar Puteri
North–South Expressway Southern Route AH2 Kuala Lumpur
Johor Premium Outlets | Multi-tier interchange |
| | Senai Masjid Bandar Senai | | |
| | Sungai Senai bridge | | |
| | Senai | | |
| 114 | Senai Senai Junctions | Taman Damai Taman Bahagia | Junctions |
| | Senai | | |
| | Senai Taman Senai | Taman Senai | Off-ramp from Johor Bahru direction on-ramp to northbound direction |
| | U-Turn | U-Turn North
FT 1 Kulai
FT 1 Senai Town Centre
FT 16 Senai International Airport  | South bound |
| | U-Turn | U-Turn South
FT 1 Skudai
FT 1 Johor Bahru
FT 5 Pontian | North bound |
FT 1 23 | 113 | Skudai-NSE Interchange |  North–South Expressway Southern Route AH2 North–South Expressway Southern Route
North
North–South Expressway Southern Route AH2 Kuala Lumpur
North–South Expressway Southern Route AH2 Melaka
Second Link Expressway Senai International Airport 
Second Link Expressway AH143 Tuas (Singapore)
South
Johor Bahru Eastern Dispersal Link Expressway AH2 Johor Bahru
Johor Bahru Eastern Dispersal Link Expressway AH2 Woodlands (Singapore)
North–South Expressway Southern Route AH2 Kempas | Cloverleaf expressway interchange |
| | Johor Technology Park Exit | West Persiaran Teknologi Johor Technology Park SIRIM Southern Region | North bound |
| | Universiti Teknologi Malaysia (UTM) (Gate 3) | Universiti Teknologi Malaysia (UTM) (GATE 3) | North bound |
Kulai-Johor Bahru district border |
| | U-Turn | U-Turn North
FT 1 Kulai
FT 1 Senai
FT 16 Senai International Airport 
 Senai–Desaru Expressway Desaru
 North–South Expressway Southern Route AH2 Kuala Lumpur | U-Turn South bound |
| | U-Turn | U-Turn South
FT 1 Skudai
FT 1 Johor Bahru
AH2 Woodlands (Singapore)
FT 17 Pasir Gudang
FT 5 Pontian | U-Turn North bound |
| | PUB-Skudai | Public Utilities Board (PUB) Singapore-Skudai water pipe maintenance facilities | South bound |
| | Skudai TNB intake | Tenaga Nasional Berhad (TNB)-Skudai intake | North bound |
FT 1 19 | 112 | Skudai North Interchange | West
FT 5 Skudai-Pontian Highway
FT 5 Pontian
FT 95 Kukup
FT 95 Tanjung Piai Universiti Teknologi Malaysia (UTM) 
East Persiaran Impian Emas Taman Impian Emas | Trumpet interchange |
| | Skudai Skudai Parade | | South bound |
| | Skudai MBIP | Iskandar Puteri City Council (MBIP) main headquarters | North bound |
| | Skudai flyover | | Start/End of flyover |
FT 1 17 | 111 | Skudai Skudai Interchange | West
J4 Jalan Gelang Patah-Skudai
J4 Taman Ungku Tun Aminah
J4 Lima Kedai
J4 Gelang Patah | Diamond interchange |
| | Skudai Taman Ungku Tun Aminah | Taman Ungku Tun Aminah | North bound |
| | Skudai flyover | | Start/End of flyover |
Sungai Skudai bridge MBIP-MBJB border limit |
| | Southern University College | Southern University College | South bound |
| | Caltex Layby | Caltex | North bound |
| | Taman Sutera Utama | Taman Sutera Utama | North bound |
| | Jalan Kempas Lama | Northeast
J193 Jalan Kempas Lama
J192 Kempas | South bound |
| 110 | Paradigm Mall Interchange | Paradigm Mall | Directional-T interchange |
| | Taman Munshi Ibrahim | Taman Munshi Ibrahim | South bound |
| | Taman Tampoi Indah | Taman Tampoi Indah Shell petrol stations | North bound |
| | Taman Johor | Taman Johor | South bound |
| 109 | Tampoi North Interchange | FT 17 Pasir Gudang Highway
West
FT 17 Taman Perling
 Second Link Expressway AH143 Second Link Expressway Pontian-Johor Bahru Parkway
Second Link Expressway Iskandar Puteri
FT 16 Senai International Airport 
North–South Expressway Southern Route AH2 Kuala Lumpur
Second Link Expressway AH143 Gelang Patah
FT 177 Port of Tanjung Pelepas 
Second Link Expressway AH143 Tuas (Singapore)
East
FT 17 Bandar Sri Alam
FT 17 Pasir Gudang
FT 177 Johor Port 
FT 3 AH18 Kota Tinggi | Double u-turn interchange |
| | Petronas Petronas Layby | Petronas McDonald's  | Southbound |
| | Giant Hypermarket Tampoi | Giant Hypermarket Tampoi | Southbound |
FT 1 10 | 108 | Tampoi Tampoi Interchange | FT 3374 Jalan Tampoi
West Pengkalan Rinting Kompleks Tan Sri Mohammad Rahmat
East Bandar Baru UDA Kempas Bandar Damansara Alif Hospital Permai Johor Bahru  | Diamond interchange |
| | Sungai Tampoi bridge | | |
| | Bandar Baru UDA | Northeast Jalan Padi Bandar Baru UDA Petronas petrol stations and southern headquarters | South bound |
| | Danga Bay flyover (towards Senai only) | | End of flyover North bound |
| 107 | Danga Bay flyover (towards Senai only)
Danga Bay Interchange | FT 52 J1 Iskandar Coastal Highway
West
FT 52 Lebuhraya Sultan Iskandar
FT 52 Iskandar Coastal Bridge
FT 52 Iskandar Puteri
FT 52 Gelang Patah
 Second Link Expressway AH143 Tuas (Singapore)
FT 52 Taman Perling
FT 52 Kota Iskandar Legoland Malaysia
Southeast
J1 Persiaran Sultan Abu Bakar Danga Bay Istana Bukit Serene Dataran Bandaraya Sultan Abu Bakar State Mosque Istana Besar Hospital Sultanah Aminah  | Interchange |
| | Danga Bay flyover (towards Senai only) | Upper Flyover
FT 1 Skudai Highway
North
FT 16 Senai International Airport 
FT 1 Senai
FT 1 Skudai
FT 1 Kulai
 North–South Expressway Southern Route AH2 Kuala Lumpur | Start of flyover Northbound |
| 106 | Taman Tasek Junctions | Taman Tasek | North bound |
| | Petronas Shell Petronas and Shell Layby | Petronas Shell  | North bound |
| 105 | RJCC Interchange | Royal Johor Country Club | Half diamond interchange |
FT 1 6 | 104 | Jalan Datin Halima Interchange | J3 Jalan Datin Halimah
North Larkin Larkin Sentral 
South Jalan Kolam Ayer Kompleks RTM Sultan Iskandar Dataran Bandaraya Taman Merdeka | Cloverleaf interchange |
| | Jalan Yahya Awal | South Jalan Yahya Awal Hutan Bandar | North bound |
| | Taman Larkin | Taman Larkin | South bound |
| | Johor Bahru Central Fire Station | Johor Bahru Central Fire Station | |
| | Jalan Kebun Teh | East Jalan Kebun Teh Jalan Dato' Jaafar Malaysian Public Works Department (JKR) Johor state Headquarters | South bound |
| 103 | Ulu Ayer Molek Interchange | West Jalan Ulu Ayer Molek Jalan Dato' Abdul Rahman Andak
Northeast Jalan Kebun Teh Lama | Junctions |
| | Danga City Mall | Danga City Mall | South bound |
| 102 | Danga City Mall-JBIRR Interchange | FT 188 Johor Bahru Inner Ring Road
West
J1 Jalan Skudai
FT 188 Dataran Bandaraya
FT 188 Jalan Yahya Awal
East
FT 1 Woodlands (Singapore)
FT 188 Stulang | Multi level Single-point urban interchange |
FT 1 1 | 101 | Johor Bahru Landmark Interchange | Northeast
FT 3 AH18 Tebrau Highway
FT 3 AH18 Mersing FT 92 Desaru
FT 3 AH18 Kota Tinggi
FT 17 Bandar Sri Alam
FT 17 Pasir Gudang
J5 Permas Jaya
FT 188 Woodlands (Singapore) | Cloverleaf interchange |
| | Komtar JBCC | | |
| | Caltex Caltex Layby | Caltex | South bound |
| | Johor Bahru City Square | | |
| | P&R JB Sentral | P&R JB Sentral Arrival/Departure
Tourist Information Centre
 KTM Intercity | |
FT 1 Skudai Highway Start/End of highway |
| | Johor Bahru old customs and immigration checkpoint | RM (Cash) | Toll operation discontinued. |
FT 1 0 | | Johor Bahru old railway station | Johor Bahru old railway station
West Jalan Station Jalan Wong Ah Fook | T-junctions |