Skyline (software)

Skyline is an open source software for targeted proteomics^[1][2] and metabolomics^[3] data analysis. It runs on Microsoft Windows and supports the raw data formats from multiple mass spectrometric vendors. It contains a graphical user interface to display chromatographic data for individual peptide or small molecule analytes.

Skyline
Developer(s)	Brendan X. MacLean et al.
Initial release	17 February 2009; 16 years ago (2009-02-17)
Stable release	21.2
Repository	github.com/ProteoWizard/pwiz/tree/master/pwiz_tools/Skyline
Written in	C#
Operating system	Windows
Type	Bioinformatics / Mass spectrometry software
License	Apache license 2.0
Website	Skyline Homepage

Skyline supports multiple workflows including selected reaction monitoring (SRM) / multiple reaction monitoring (MRM),^[4] parallel reaction monitoring (PRM),^[5][6] data-independent acquisition (DIA/SWATH)^[7] and targeted data-dependent acquisition.^[8]

See also

• ProteoWizard
• OpenMS
• Trans-Proteomic Pipeline
• Mass spectrometry software