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Spot-tag
From Wikipedia, the free encyclopedia
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A Spot-tag is a 12-amino acid peptide tag recognized by a single-domain antibody (sdAb, or nanobody). Due to the small size of a Spot-tag (12 amino acids) and the robust Spot-nanobody (14.7 kD) that specifically binds to Spot-tagged proteins, Spot-tag can be used for multiple capture and detection applications: Immunoprecipitation, affinity purification, immunofluorescence, and super-resolution microscopy.[1] Recombinant proteins can be engineered to express the Spot-tag.
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Spot-tag Sequence
Amino acid sequence
PDRVRAVSHWSS
Codon optimized DNA sequence
| Human | CCA GAC CGC GTG CGC GCC GTG AGC CAT TGG AGC AGC |
| S. cerevisiae | CCA GAT AGA GTT AGA GCT GTT TCT CAT TGG TCT TCT |
| E. coli | CCG GAT CGC GTG CGC GCA GTC TCT CAC TGG AGC AGC |
See also
References
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