TRNAIle-lysidine synthase

TRNAIle-lysidine synthase (EC 6.3.4.19, TilS, mesJ (gene), yacA (gene), isoleucine-specific transfer ribonucleate lysidine synthetase, tRNAIle-lysidine synthetase) is an enzyme with systematic name L-lysine:(tRNAIle2)-cytidine34 ligase (AMP-forming).^{[1][2][3][4][5]} This enzyme catalyses the following chemical reaction

[tRNAIle2]-cytidine34 + L-lysine + ATP ${\displaystyle \rightleftharpoons }$ [tRNAIle2]-lysidine³⁴ + AMP + diphosphate + H₂O

TRNAIle-lysidine synthase
Identifiers
EC no.	6.3.4.19
CAS no.	635304-92-6
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

The bacterial enzyme modifies the wobble base of the CAU anticodon of tRNAIle at the oxo group in position 2 of cytidine³⁴.