TRNA pseudouridine32 synthase

tRNA pseudouridine³² synthase (EC 5.4.99.28, RluA, pseudouridine synthase RluA, Pus9p, Rib2/Pus8p) is an enzyme with systematic name tRNA-uridine³² uracil mutase.^{[1][2][3][4][5][6]} This enzyme catalyses the following chemical reaction

tRNA uridine³² ${\displaystyle \rightleftharpoons }$ tRNA pseudouridine³²

tRNA pseudouridine32 synthase
Identifiers
EC no.	5.4.99.28
CAS no.	430429-15-5
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

The dual enzyme from Escherichia coli also catalyses the formation of pseudouridine⁷⁴⁶ in 23S rRNA.