Thiocyanate hydrolase

A thiocyanate hydrolase (EC 3.5.5.8) is an enzyme belonging to the family of hydrolases. The systematic name of this enzyme class is thiocyanate aminohydrolase. This enzyme catalyzes the chemical reaction:

SCN⁻ + 2 H₂O + H⁺ ⇌ SCO + NH₃

thiocyanate hydrolase
Identifiers
EC no.	3.5.5.8
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

The mechanism is proposed to involve a metal thiocyanate complex.

Structural studies

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 2DD4, 2DD5, 2DXB, and 2DXC.

A second thiocyanate hydrolase with copper at its active site catalyzes its conversion to cyanate:^[1]

SCN⁻ + H₂O → OCN⁻ + H₂S