Toluene dioxygenase

In enzymology, a toluene dioxygenase (EC 1.14.12.11) is an enzyme that catalyzes the chemical reaction

toluene + NADH + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD⁺

toluene dioxygenase
Identifiers
EC no.	1.14.12.11
CAS no.	120038-36-0
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

The 4 substrates of this enzyme are toluene, NADH, H⁺, and O₂, whereas its two products are (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol and NAD⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of two atoms o oxygen into the other donor. The systematic name of this enzyme class is toluene,NADH:oxygen oxidoreductase (1,2-hydroxylating). This enzyme is also called toluene 2,3-dioxygenase. This enzyme participates in toluene and xylene degradation.