Tombusvirus 5′ UTR

Tombusvirus 5′ UTR is an important cis-regulatory region of the Tombus virus genome.

Tombusvirus 5′ UTR
Predicted secondary structure and sequence conservation of Tombus_5
Identifiers
Symbol	Tombus_5
Rfam	RF00171
Other data
RNA type	Cis-reg
Domain(s)	Viruses
SO	SO:0000204
PDB structures	PDBe

Tomato bushy stunt virus is the prototype member of the Tombusviridae family. The genome of this virus is positive sense single stranded RNA. Replication occurs via a negative strand RNA intermediate. In addition to viral proteins p33 and the RNA-dependent RNA polymerase p92, and unknown host factors, conserved and structural regions within the 5′ untranslated region (5′ UTR) are important for regulating genome replication.^{[citation needed]}

2 RNA domains in the 5′ UTR have been reported, a 5′ T-shaped domain (TSD) followed by a stem-loop (SL5)^[1][2] and a downstream domain (DSD).^[3] TSD-DSD interactions are proposed to be involved in the mediation of viral RNA replication.^[3]

An interesting feature of Tombusvirus is its ability to support the replication of defective interfering (DI) RNAs. These sub-viral replicons are small, non-coding, deletion mutants of the viral genome that maintain cis-acting RNA elements necessary for replication ^[1]

Other non-coding RNA structures in Tombusvirus include the 3′ UTR region IV^[4] and an internal replication element.^[5]