| Sija | Erä | Rata | Maa | Urheilijat | Aika | Huom. |
| 1 | 1 | 4 |  Yhdysvallat | Tyrell Richard, Vernon Norwood, Wil London, Nathan Strother | 2.59,89 | Q |
| 2 | 2 | 5 |  Jamaika | Akeem Bloomfield, Nathon Allen, Terry Thomas, Javon Francis | 3.00,76 | Q, SB |
| 3 | 2 | 6 |  Belgia | Dylan Borlée, Julien Watrin, Jonathan Sacoor, Kevin Borlée | 3.00,87 | Q, SB |
| 4 | 1 | 9 |  Kolumbia | Jhon Perlaza, Diego Palomeque, Jhon Solís, Anthony Zambrano | 3.01,06 | Q, NR |
| 5 | 2 | 8 |  Trinidad ja Tobago | Asa Guevara, Jereem Richards, Darren Alfred, Deon Lendore | 3.01,35 | Q |
| 6 | 2 | 7 |  Ranska | Ludvy Vaillant, Christopher Naliali, Thomas Jordier, Mame-Ibra Anne | 3.01,40 | q, SB |
| 7 | 1 | 8 |  Italia | Edoardo Scotti, Vladimir Aceti, Matteo Galvan, Davide Re | 3.01,60 | Q, SB |
| 8 | 1 | 6 |  Iso-Britannia | Cameron Chalmers, Rabah Yousif, Lee Thompson, Martyn Rooney | 3.01,96 | q, SB |
| 9 | 1 | 7 |  Japani | Julian Walsh, Shōta Iizuka, Kentaro Sato, Kota Wakabayashi | 3.02,05 | SB |
| 10 | 2 | 3 |  Etelä-Afrikka | Thapelo Phora, Gardeo Isaacs, Ranti Dikgale, Derrick Mokaleng | 3.02,06 | SB |
| 11 | 2 | 9 |  Tšekki | Jan Tesař, Michal Desenský, Patrik Šorm, Vít Müller | 3.02,97 | SB |
| 12 | 2 | 4 |  Intia | Amoj Jacob, Muhammed Anas, Jeevan Karekoppa Suresh, Noah Nirmal Tom | 3.03,09 | |
| 13 | 1 | 3 |  Espanja | Óscar Husillos, Samuel García, Julio Arenas, Darwin Echeverry | 3.04,27 | SB |
| 14 | 1 | 2 |  Australia | Steven Solomon, Alex Beck, Murray Goodwin, Ian Halpin | 3.05,49 | |
| 15 | 2 | 2 |  Qatar | Bassem Hemeida, Abubaker Haydar Abdalla, Ashraf Hussein Osman, Mohamed Nasir Abbas | 3.06,25 | |
| 16 | 1 | 5 |  Botswana | Zibane Ngozi, Ditiro Nzamani, Onkabetse Nkobolo, Leungo Scotch | 9.99,99 DQ | 170.19 |
