Acetyl-CoA C-acetyltransferase

Not to be confused with Acyl-CoA:cholesterol acyltransferase or Acetyl-CoA C-acyltransferase.

In enzymology, an acetyl-CoA C-acetyltransferase (EC 2.3.1.9) is an enzyme that catalyzes the chemical reaction

2 acetyl-CoA ${\displaystyle \rightleftharpoons }$ CoA + acetoacetyl-CoA

acetyl-CoA C-acetyltransferase
Crystallographic structure of the tetrameric acetoacetyl-CoA thiolase (EC:2.3.1.9) from the Gram-negative bacterium Z. ramigera.[1]
Identifiers
EC no.	2.3.1.9
CAS no.	9027-46-7
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Hence, this enzyme has one substrate, acetyl-CoA, and two products, CoA and acetoacetyl-CoA.

Acetyl-CoA C-acetyltransferase belongs to the thiolase family of enzymes.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acetyl-CoA:acetyl-CoA C-acetyltransferase. Other names in common use include acetoacetyl-CoA thiolase, beta-acetoacetyl coenzyme A thiolase, 2-methylacetoacetyl-CoA thiolase [misleading], 3-oxothiolase, acetyl coenzyme A thiolase, acetyl-CoA acetyltransferase, acetyl-CoA:N-acetyltransferase, and thiolase II. This enzyme participates in 10 metabolic pathways: fatty acid metabolism, synthesis and degradation of ketone bodies, valine, leucine and isoleucine degradation, lysine degradation, tryptophan metabolism, pyruvate metabolism, benzoate degradation via coa ligation, propanoate metabolism, butanoate metabolism, and two-component system - general.

Isozymes

Human genes encoding acetyl-CoA C-acetyltransferases include:

acetyl-Coenzyme A acetyltransferase 1 (acetoacetyl Coenzyme A Thiolase) Identifiers Symbol ACAT1 NCBI gene 38 HGNC 93 OMIM 607809 RefSeq NM_000019 UniProt P24752 Other data EC number 2.3.1.9 Locus Chr. 11 q22.3-23.1 Search for Structures Swiss-model Domains InterPro

acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase) Identifiers Symbol ACAT2 NCBI gene 39 HGNC 94 OMIM 100678 RefSeq NM_005891 UniProt Q9BWD1 Other data EC number 2.3.1.9 Locus Chr. 6 q25.3-q26 Search for Structures Swiss-model Domains InterPro