CKS1B

Cyclin-dependent kinases regulatory subunit 1 is a protein that in humans is encoded by the CKS1B gene.^[5][6]

CKS1B
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1BUH, 1DKS, 1DKT, 2ASS, 2AST, 4YC6
Identifiers
Aliases	CKS1B, CKS1, PNAS-16, PNAS-18, ckshs1, CDC28 protein kinase regulatory subunit 1B
External IDs	OMIM: 116900; MGI: 1889208; HomoloGene: 123798; GeneCards: CKS1B; OMA:CKS1B - orthologs
Gene location (Human) Chr. Chromosome 1 (human)[1] Band 1q21.3 Start 154,974,653 bp[1] End 154,979,251 bp[1]
Gene location (Mouse) Chr. Chromosome 3 (mouse)[2] Band 3|3 F1 Start 89,322,779 bp[2] End 89,325,690 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in ventricular zone ganglionic eminence lymph node mucosa of transverse colon placenta rectum testicle subcutaneous adipose tissue gonad mucosa of esophagus Top expressed in endocardial cushion mandibular prominence maxillary prominence ureter medullary collecting duct renal corpuscle somite atrioventricular valve Gonadal ridge abdominal wall More reference expression data BioGPS n/a
Gene ontology Molecular function cyclin-dependent protein serine/threonine kinase regulator activity protein binding histone binding protein kinase binding cyclin-dependent protein serine/threonine kinase activator activity ubiquitin binding Cellular component nucleoplasm SCF ubiquitin ligase complex cyclin-dependent protein kinase holoenzyme complex Biological process cell cycle cell division regulation of mitotic cell cycle positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of transcription, DNA-templated regulation of transcription, DNA-templated cell population proliferation mitotic cell cycle phase transition regulation of cyclin-dependent protein serine/threonine kinase activity Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1163 54124 Ensembl ENSG00000173207 ENSMUSG00000028044 UniProt P61024 P61025 RefSeq (mRNA) NM_001826 NM_016904 RefSeq (protein) NP_001817 NP_058600 Location (UCSC) Chr 1: 154.97 – 154.98 Mb Chr 3: 89.32 – 89.33 Mb PubMed search [3] [4]
Wikidata
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Function

The CKS1B protein binds to the catalytic subunit of the cyclin-dependent kinases and is essential for their biological function. The CKS1B mRNA is found to be expressed in different patterns through the cell cycle in HeLa cells, which reflects a specialized role for the encoded protein.^[6]

CKS1B and CKS2 proteins have demonstrated principal roles in cell cycle regulation. Defined originally as suppressors of mutations in both fission and budding yeast Cdk1 genes, Cks molecules interact with Cdk1, Cdk2 and Cdk3. These Cdk-dependent enzyme complexes in cell cycle regulation frequently consist of Cdk molecules bound to a catalytic Cdk subunit, i.e. Cks and a regulatory cyclin subunit, such as a G1 cyclin, controlling Cdk function by directing cyclin-cdk complex activity toward specific and significant substrates. Malfunctions of cdk-dependent associations lead to defects into the entry of mitosis for cells.^[7]

Cks1 in the Cdk-independent pathway involves the recognition of substrates p27^Kip1 and p21^cip1 by directly associating with E3 SCF^Skp2 when stimulated by certain mitogenic signals, such as TGF-β.^[8]

Clinical significance

Cks1-depleted breast cancer cells not only exhibit slowed G(1) progression, but also accumulate in G(2)-M due to blocked mitotic entry. Cdk1 expression, which is crucial for M phase entry, is drastically diminished by Cks1 depletion, and that restoration of cdk1 reduces G(2)-M accumulation in Cks1-depleted cells.^[9]

Interactions

CKS1B has been shown to interact with SKP2^{[10][11][12][13]} and CDKN1B.^[10][11]