DAD1

Dolichyl-diphosphooligosaccharide—protein glycosyltransferase subunit DAD1 is an enzyme that in humans is encoded by the DAD1 gene.^[5]

DAD1
Identifiers
Aliases	DAD1, OST2, defender against cell death 1
External IDs	OMIM: 600243; MGI: 101912; HomoloGene: 1027; GeneCards: DAD1; OMA:DAD1 - orthologs
Gene location (Human) Chr. Chromosome 14 (human)[1] Band 14q11.2 Start 22,564,907 bp[1] End 22,589,224 bp[1]
Gene location (Mouse) Chr. Chromosome 14 (mouse)[2] Band 14 C2|14 27.7 cM Start 54,472,936 bp[2] End 54,491,561 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in islet of Langerhans gallbladder right lobe of thyroid gland anterior pituitary left lobe of thyroid gland right adrenal gland canal of the cervix rectum left adrenal gland stromal cell of endometrium Top expressed in choroid plexus of fourth ventricle facial motor nucleus seminal vesicula islet of Langerhans fossa internal carotid artery efferent ductule adrenal gland external carotid artery carotid body More reference expression data BioGPS More reference expression data
Gene ontology Molecular function dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyl transferase activity Cellular component integral component of membrane oligosaccharyltransferase complex extracellular exosome endoplasmic reticulum membrane membrane endoplasmic reticulum Biological process protein glycosylation negative regulation of apoptotic process protein N-linked glycosylation via asparagine blastocyst development response to nutrient apoptotic process protein N-linked glycosylation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1603 13135 Ensembl ENSG00000129562 ENSMUSG00000022174 UniProt P61803 P61804 RefSeq (mRNA) NM_001344 NM_001113358 NM_010015 RefSeq (protein) NP_001335 NP_001106829 NP_034145 Location (UCSC) Chr 14: 22.56 – 22.59 Mb Chr 14: 54.47 – 54.49 Mb PubMed search [3] [4]
Wikidata
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Function

DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes.^[5]

Interactions

DAD1 has been shown to interact with MCL1.^[6]