GRLF1

Glucocorticoid receptor DNA-binding factor 1 is a protein that in humans is encoded by the GRLF1 gene.^[5][6]

ARHGAP35
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2K85, 3C5H, 3FK2
Identifiers
Aliases	ARHGAP35, GRF-1, GRLF1, P190-A, P190A, p190ARhoGAP, p190RhoGAP, Rho GTPase activating protein 35
External IDs	OMIM: 605277; MGI: 1929494; HomoloGene: 35136; GeneCards: ARHGAP35; OMA:ARHGAP35 - orthologs
Gene location (Human) Chr. Chromosome 19 (human)[1] Band 19q13.32 Start 46,860,997 bp[1] End 47,005,077 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7|7 A2 Start 16,227,644 bp[2] End 16,348,918 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Brodmann area 23 endothelial cell middle temporal gyrus postcentral gyrus entorhinal cortex superior vestibular nucleus left testis pars compacta superior frontal gyrus right testis Top expressed in saccule otic placode genital tubercle otic vesicle tail of embryo dentate gyrus of hippocampal formation granule cell visual cortex primary visual cortex superior frontal gyrus secondary oocyte More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription corepressor activity GTP binding DNA-binding transcription repressor activity, RNA polymerase II-specific phospholipid binding GTPase activator activity GTPase activity nucleotide binding lipid binding Cellular component cytoplasm cytosol actin cytoskeleton nucleus cytoskeleton cell projection plasma membrane membrane ciliary basal body Biological process regulation of transcription, DNA-templated negative regulation of Rho protein signal transduction negative regulation of transcription by RNA polymerase II transcription, DNA-templated neural tube closure regulation of cell shape forebrain development camera-type eye development negative regulation of transcription, DNA-templated regulation of small GTPase mediated signal transduction signal transduction negative regulation of vascular permeability positive regulation of GTPase activity axon guidance axonal fasciculation regulation of actin polymerization or depolymerization positive regulation of neuron projection development cell migration central nervous system neuron axonogenesis mammary gland development establishment or maintenance of actin cytoskeleton polarity cellular response to extracellular stimulus regulation of actin cytoskeleton organization wound healing, spreading of cells positive regulation of cilium assembly regulation of axonogenesis neuron projection guidance Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 2909 232906 Ensembl ENSG00000160007 ENSMUSG00000058230 UniProt Q9NRY4 Q91YM2 RefSeq (mRNA) NM_004491 NM_024342 NM_172739 RefSeq (protein) NP_004482 NP_766327 Location (UCSC) Chr 19: 46.86 – 47.01 Mb Chr 7: 16.23 – 16.35 Mb PubMed search [3] [4]
Wikidata
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Function

The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids.^[6]