GRWD1

Glutamate-rich WD repeat-containing protein 1 is a WD40 repeat protein (containing five WD40 repeat motifs) that in humans is encoded by the GRWD1 gene. It localizes to the nucleus and has known functions in regulating chromatin accessibility and loading of the MCM helicase.^[5][6] GRWD1 has also been shown to play a critical role in ribosome biogenesis.^[5]

GRWD1
Identifiers
Aliases	GRWD1, CDW4, GRWD, RRB1, WDR28, glutamate-rich WD repeat containing 1, glutamate rich WD repeat containing 1
External IDs	OMIM: 610597; MGI: 2141989; HomoloGene: 6644; GeneCards: GRWD1; OMA:GRWD1 - orthologs
Gene location (Human) Chr. Chromosome 19 (human)[1] Band 19q13.33 Start 48,445,841 bp[1] End 48,457,022 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7|7 B3 Start 45,474,647 bp[2] End 45,480,368 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in gastrocnemius muscle stromal cell of endometrium olfactory bulb gonad muscle of thigh mucosa of esophagus left uterine tube skin of leg apex of heart granulocyte Top expressed in internal carotid artery external carotid artery yolk sac epiblast condyle primitive streak fossa somite endothelial cell of lymphatic vessel embryo More reference expression data BioGPS More reference expression data
Gene ontology Molecular function chromatin binding DNA replication origin binding RNA binding protein binding histone binding Cellular component nucleus nucleolus cytosol chromosome protein-containing complex Biological process nucleosome assembly nucleosome disassembly DNA replication Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 83743 101612 Ensembl ENSG00000105447 ENSMUSG00000053801 UniProt Q9BQ67 Q810D6 RefSeq (mRNA) NM_031485 NM_153419 RefSeq (protein) NP_113673 NP_700468 Location (UCSC) Chr 19: 48.45 – 48.46 Mb Chr 7: 45.47 – 45.48 Mb PubMed search [3] [4]
Wikidata
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Role in cancer

While some ribosomal proteins like RPL5 and RPL11 are suggested to act as tumor suppressors by inhibiting E3 ubiquitin-protein ligase MDM2 and thus activating p53, others, such as GRWD1, may promote tumorigenesis. Overexpression of GRWD1 suppresses p53 and transforms normal cells, possibly through its interaction with RPL11, preventing it from regulating MDM2.^[7]

In addition to its interaction with RPL11, GRWD1 directly interacts with wild-type p53, suppressing its transcriptional activity.^[8] Furthermore, overexpression of GRWD1 has been linked to the activation of oncogenic signaling pathways, such as the Notch signaling pathway, through the upregulation of ADAM17.^[9]