Lipase

Not to be confused with Ligase.

Lipase is a class of enzymes that catalyzes the hydrolysis of fats. Some lipases display broad substrate scope including esters of cholesterol, phospholipids, and of lipid-soluble vitamins^[1][2] and sphingomyelinases;^[3] however, these are usually treated separately from "conventional" lipases. Unlike esterases, which function in water, lipases "are activated only when adsorbed to an oil–water interface".^[4] Lipases perform essential roles in digestion, transport and processing of dietary lipids in most, if not all, organisms.

Lipase
A computer-generated image of a type of pancreatic lipase (PLRP2) from the guinea pig. PDB: 1GPL​
Pronunciation	/ˈlaɪpeɪs, ˈlaɪpeɪz/ LY-payss, LY-payz
Test of	Pancreatitis
Conversion calculator SI (µkat/L) Conventional (U/L)

Structure and catalytic mechanism

Classically, lipases catalyse the hydrolysis of triglycerides:^{[citation needed]} $${\displaystyle {\begin{aligned}{\text{triglyceride}}+{\ce {H2O}}&\longrightarrow {\text{fatty acid}}+{\text{diacylglycerol}}\\[4pt]{\text{diacylglycerol}}+{\ce {H2O}}&\longrightarrow {\text{fatty acid}}+{\text{monacylglycerol}}\\[4pt]{\text{monacylglycerol}}+{\ce {H2O}}&\longrightarrow {\text{fatty acid}}+{\text{glycerol}}\end{aligned}}}$$

Lipases are serine hydrolases, i.e. they function by transesterification generating an acyl serine intermediate. Most lipases act at a specific position on the glycerol backbone of a lipid substrate (A1, A2 or A3). For example, human pancreatic lipase (HPL),^[5] converts triglyceride substrates found in ingested oils to monoglycerides and two fatty acids.

A diverse array of genetically distinct lipase enzymes are found in nature, and they represent several types of protein folds and catalytic mechanisms. However, most are built on an alpha/beta hydrolase fold^[6][7][8][9] and employ a chymotrypsin-like hydrolysis mechanism using a catalytic triad consisting of a serine nucleophile, a histidine base, and an acid residue, usually aspartic acid.^[10][11]

Physiological distribution

Lipases are involved in diverse biological processes which range from routine metabolism of dietary triglycerides to cell signaling^[12] and inflammation.^[13] Thus, some lipase activities are confined to specific compartments within cells while others work in extracellular spaces.

• In the example of lysosomal lipase, the enzyme is confined within an organelle called the lysosome.
• Other lipase enzymes, such as pancreatic lipases, are secreted into extracellular spaces where they serve to process dietary lipids into more simple forms that can be more easily absorbed and transported throughout the body.
• Fungi and bacteria may secrete lipases to facilitate nutrient absorption from the external medium (or in examples of pathogenic microbes, to promote invasion of a new host).
• Certain wasp and bee venoms contain phospholipases that enhance the effects of injury and inflammation delivered by a sting.
• As biological membranes are integral to living cells and are largely composed of phospholipids, lipases play important roles in cell biology.
• Malassezia globosa, a fungus thought to be the cause of human dandruff, uses lipase to break down sebum into oleic acid and increase skin cell production, causing dandruff.^[14]

Genes encoding lipases are even present in certain viruses.^[15][16]

Some lipases are expressed and secreted by pathogenic organisms during an infection. In particular, Candida albicans has many lipases, possibly reflecting broad-lipolytic activity, which may contribute to the persistence and virulence of C. albicans in human tissue.^[17]

Human lipases

Name	Gene	Location	Description	Disorder
bile salt-dependent lipase	BSDL	pancreas, breast milk	aids in the digestion of fats[1]
pancreatic lipase	PNLIP	digestive juice	Human pancreatic lipase (HPL) is the main enzyme that breaks down dietary fats in the human digestive system.[5] To exhibit optimal enzyme activity in the gut lumen, PL requires another protein, colipase, which is also secreted by the pancreas.[18]
lysosomal lipase	LIPA	interior space of organelle: lysosome	Also referred to as lysosomal acid lipase (LAL or LIPA) or acid cholesteryl ester hydrolase	Cholesteryl ester storage disease (CESD) and Wolman disease are both caused by mutations in the gene encoding lysosomal lipase.[19]
hepatic lipase	LIPC	endothelium	Hepatic lipase acts on the remaining lipids carried on lipoproteins in the blood to regenerate LDL (low density lipoprotein).	–
lipoprotein lipase	LPL or "LIPD"	endothelium	Lipoprotein lipase functions in the blood to act on triacylglycerides carried on VLDL (very low density lipoprotein) so that cells can take up the freed fatty acids.	Lipoprotein lipase deficiency is caused by mutations in the gene encoding lipoprotein lipase.[20][21]
hormone-sensitive lipase	LIPE	intracellular	–	–
gastric lipase	LIPF	digestive juice	Functions in the infant at a near-neutral pH to aid in the digestion of lipids	–
endothelial lipase	LIPG	endothelium	–	–
pancreatic lipase related protein 2	PNLIPRP2 or "PLRP2" –	digestive juice	–	–
pancreatic lipase related protein 1	PNLIPRP1 or "PLRP1"	digestive juice	Pancreatic lipase related protein 1 is very similar to PLRP2 and PL by amino acid sequence (all three genes probably arose via gene duplication of a single ancestral pancreatic lipase gene). However, PLRP1 is devoid of detectable lipase activity and its function remains unknown, even though it is conserved in other mammals.[22][23]	-
lingual lipase	?	saliva	Active at gastric pH levels. Optimum pH is about 3.5-6. Secreted by several of the salivary glands (Ebner's glands at the back of the tongue (lingua), the sublingual glands, and the parotid glands)	–

Other lipases include LIPH, LIPI, LIPJ, LIPK, LIPM, LIPN, MGLL, DAGLA, DAGLB, and CEL.

Uses

In the commercial sphere, lipases are widely used in laundry detergents. Several thousand tons per year are produced for this role.^[4]

Lipases are catalysts for hydrolysis of esters and are useful outside of the cell, a testament to their wide substrate scope and ruggedness. The ester hydrolysis activity of lipases has been well evaluated for the conversion of triglycerides into biofuels or their precursors.^{[24][25][26][27]}

Lipases are chiral, which means that they can be used for the enantioselective hydrolysis prochiral diesters.^[28] Several procedures have been reported for applications in the synthesis of fine chemicals.^[29][30][31]

Lipases are generally animal sourced, but can also be sourced microbially.^{[citation needed]}

Biomedicine

Blood tests for lipase may be used to help investigate and diagnose acute pancreatitis and other disorders of the pancreas.^[32] Measured serum lipase values may vary depending on the method of analysis.^{[citation needed]}

Lipase assist in the breakdown of fats in those undergoing pancreatic enzyme replacement therapy (PERT). It is a component in Sollpura (Liprotamase).^[33][34]

See also

• Alpha toxin
• Pathology
• Lysosomal acid lipase deficiency
• Peripheral membrane proteins
• Phospholipase A
• Phospholipase C
• Triglyceride lipase
• Phospholipase A2
• Outer membrane phospholipase A1
• Patatin-like phospholipase