MID2

Midline-2 is a protein that in humans is encoded by the MID2 gene.^[5][6]

MID2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2DJA, 2DMK
Identifiers
Aliases	MID2, FXY2, MRX101, RNF60, TRIM1, midline 2, XLID101
External IDs	OMIM: 300204; MGI: 1344333; HomoloGene: 8028; GeneCards: MID2; OMA:MID2 - orthologs
Gene location (Human) Chr. X chromosome (human)[1] Band Xq22.3 Start 107,825,755 bp[1] End 107,931,637 bp[1]
Gene location (Mouse) Chr. X chromosome (mouse)[2] Band X F1|X 61.35 cM Start 139,565,348 bp[2] End 139,668,464 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in tibialis anterior muscle islet of Langerhans cartilage tissue muscle of thigh smooth muscle tissue gastrocnemius muscle skin of thigh pancreatic ductal cell popliteal artery tibial arteries Top expressed in lateral nasal prominence medial nasal prominence left lung lobe supraoptic nucleus female urethra superior frontal gyrus dentate gyrus of hippocampal formation granule cell genital tubercle ascending aorta cerebellar cortex More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein homodimerization activity microtubule binding zinc ion binding metal ion binding phosphoprotein binding protein binding protein heterodimerization activity identical protein binding transferase activity Cellular component cytoplasm intracellular anatomical structure microtubule extracellular exosome cytoskeleton Biological process positive regulation of autophagy positive regulation of DNA-binding transcription factor activity protein localization to microtubule positive regulation of NF-kappaB transcription factor activity protein ubiquitination positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response negative regulation of viral transcription negative regulation of viral entry into host cell Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 11043 23947 Ensembl ENSG00000080561 ENSMUSG00000000266 UniProt Q9UJV3 Q9QUS6 RefSeq (mRNA) NM_012216 NM_052817 NM_001382751 NM_001382752 NM_011845 NM_001358366 NM_001358367 RefSeq (protein) NP_036348 NP_438112 NP_001369680 NP_001369681 NP_035975 NP_001345295 NP_001345296 NP_001390295 Location (UCSC) Chr X: 107.83 – 107.93 Mb Chr X: 139.57 – 139.67 Mb PubMed search [3] [4]
Wikidata
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Function

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Its function has not been identified. Alternate splicing of this gene results in two transcript variants encoding different isoforms.^[6]

Recent reports indicate the involvement of MID2 in cytokinesis ^[7][8].MID2 (TRIM1) ubiquitinates Sperm-associated antigen 5 (Astrin) on K409, further promoting its degradation and proper cytokinesis.^[8] In contrary, depletion of MID2 (TRIM1) stabilizes Sperm-associated antigen 5 (Astrin) whose inappropriate accumulation at the midbody triggers cytokinetic arrest, multinucleated cells, and cell death.^[7][8]

Interactions

MID2 has been shown to interact with MID1.^[9][10]

MID2 (TRIM1) interacts with Leucine-rich repeat kinase 2 (LRRK2), which is often subject to missense mutations in familial Parkinson's disease (PD).^[11] MID2 (TRIM1) specifically binds to the flexible regulatory loop of LRRK2_853–981.^[11] MID2 (TRIM1) recruits LRRK2 to the microtubule cytoskeleton where MID2 (TRIM1) ubiquitinates LRRK2 targeting it for proteasomal degradation.^[11]