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Rhodamine 6G

Chemical compound From Wikipedia, the free encyclopedia

Rhodamine 6G
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Rhodamine 6G /ˈrdəmn/ is a highly fluorescent rhodamine family dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.

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Forms

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Rhodamine 6G chloride powder mixed with methanol, emitting yellow light under green laser illumination

Rhodamine 6G usually comes in three different forms. Rhodamine 6G chloride is a bronze/red powder with the chemical formula C28H31ClN2O3. Although highly soluble, this formulation is very corrosive to all metals except stainless steel. Other formulations are less soluble, but also less corrosive. Rhodamine 6G perchlorate (C28H31ClN2O7) comes in the form of red crystals, while rhodamine 6G tetrafluoroborate (C28H31BF4N2O3) appears as maroon crystals.[1]

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Solubility

Butanol (40 g/L), ethanol (80 g/L), methanol (400 g/L), propanol (15 g/L), MEG (50 g/L), DEG ( 100 g/L), TEG (100 g/L), isopropanol (15 g/L), ethoxyethanol (25 g/L), methoxyethanol (50 g/L), dipropylene glycol (30 g/L), PEG (20 g/L).[2]

Laser dye

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Rhodamine 6G-based dye laser. The dye solution is the orange fluid in the tubes.

Rhodamine 6G is also used as a laser dye, or gain medium, in dye lasers,[3][4] and is pumped by the second (532 nm) harmonic from an Nd:YAG laser, nitrogen laser, or argon ion laser.[5] The dye has a remarkably high photostability, high fluorescence quantum yield (0.95[6]), low cost, and its lasing range has close proximity to its absorption maximum (approximately 530 nm). The lasing range of the dye is 570 to 660 nm with a maximum at 590 nm.[7]

Properties

The refractive index of Rhodamine 6G has been determined in recent studies. According to research published in ACS Applied Materials & Interfaces[8] and the Refractive Index Database,[9] the refractive index is 1.87 (k=0.79) for 532 nm wavelength.

See also

References

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