Tight junction protein ZO-1

Tight junction protein ZO-1 also known as Zonula Occludens-1 (ZO-1), is a 220-kD peripheral membrane protein that is encoded by the TJP1 gene in humans.^[5] It belongs to the family of zonula occludens proteins (ZO-1, ZO-2, and ZO-3), which are tight junction-associated proteins and of which, ZO-1 is the first to be cloned. It was first isolated in 1986 by Stevenson and Goodenough using a monoclonal antibody raised in rodent liver to recognise a 225-kD polypeptide in whole liver homogenates and in tight junction-enriched membrane fractions.^[6] It has a role as a scaffold protein which cross-links and anchors Tight Junction (TJ) strand proteins, which are fibril-like structures within the lipid bilayer, to the actin cytoskeleton.^[7]

TJP1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2H2B, 2H2C, 2H3M, 2JWE, 2KXR, 2KXS, 2RCZ, 3CYY, 3LH5, 3SHU, 3SHW, 3TSV, 3TSW, 3TSZ, 4OEO, 4OEP, 4Q2Q, 4YYX
Identifiers
Aliases	TJP1, ZO-1, Tight junction protein 1
External IDs	OMIM: 601009; MGI: 98759; HomoloGene: 2445; GeneCards: TJP1; OMA:TJP1 - orthologs
Gene location (Human) Chr. Chromosome 15 (human)[1] Band 15q13.1 Start 29,699,367 bp[1] End 29,968,915 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7 C|7 35.02 cM Start 64,945,913 bp[2] End 65,177,529 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in corpus callosum sural nerve placenta tibial arteries right lung smooth muscle tissue Achilles tendon endometrium epithelium of colon C1 segment Top expressed in left lung lobe vestibular sensory epithelium gastrula vestibular membrane of cochlear duct utricle stria vascularis external carotid artery cumulus cell epithelium of lens iris More reference expression data BioGPS More reference expression data
Gene ontology Molecular function calmodulin binding protein binding cadherin binding protein domain specific binding connexin binding protein C-terminus binding Cellular component membrane bicellular tight junction plasma membrane basolateral plasma membrane gap junction apical junction complex cytosol cytoplasm apical part of cell cell junction podosome cell projection nucleus cell-cell junction adherens junction intercalated disc apical plasma membrane apicolateral plasma membrane intercellular canaliculus tight junctions protein-containing complex Biological process establishment of endothelial intestinal barrier hippo signaling cell-cell junction assembly regulation of bicellular tight junction assembly blastocyst formation hearing response to lipopolysaccharide negative regulation of vascular permeability response to ethanol response to magnetism cellular response to glucose stimulus Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 7082 21872 Ensembl ENSG00000277401 ENSG00000104067 ENSMUSG00000030516 UniProt Q07157 P39447 RefSeq (mRNA) NM_001301025 NM_001301026 NM_003257 NM_175610 NM_001330239 NM_001355012 NM_001355013 NM_001355014 NM_001355015 NM_001163574 NM_009386 RefSeq (protein) NP_001287954 NP_001287955 NP_001317168 NP_003248 NP_783297 NP_001341941 NP_001341942 NP_001341943 NP_001341944 NP_001287954.1 NP_001287955.1 NP_001157046 NP_033412 Location (UCSC) Chr 15: 29.7 – 29.97 Mb Chr 7: 64.95 – 65.18 Mb PubMed search [3] [4]
Wikidata
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Not to be confused with Occludin.

Function

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell–cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene.^[8]

Interactions

Tight junction protein 1 has been shown to interact with:

• F11 receptor,^[9][10]
• GJA3,^[11]
• GJA8,^[11]
• Gap junction protein, alpha 1,^[12]
• KIRREL,^[13][14]
• MLLT4,^[15]
• Occludin,^[16][17]
• TJP3,^[18] and
• Tight junction protein 2.^[16][18][19]

See also

• Tight junction