ZNF184

Zinc finger protein 184, also known as ZNF184, is a protein that in humans is encoded by the ZNF184 gene^[5] on chromosome 6. It was first identified by Goldwurm et al. in 1996.^[6]

ZNF184
Identifiers
Aliases	ZNF184, kr-ZNF3, zinc finger protein 184
External IDs	OMIM: 602277; MGI: 1922244; HomoloGene: 113602; GeneCards: ZNF184; OMA:ZNF184 - orthologs
Gene location (Human) Chr. Chromosome 6 (human)[1] Band 6p22.1 Start 27,450,743 bp[1] End 27,473,118 bp[1]
Gene location (Mouse) Chr. Chromosome 13 (mouse)[2] Band 13|13 A3.1 Start 22,129,264 bp[2] End 22,144,949 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Brodmann area 23 sperm hair follicle middle temporal gyrus endothelial cell epithelium of nasopharynx germinal epithelium ganglionic eminence gingival epithelium gonad Top expressed in genital tubercle spermatid spermatocyte tail of embryo ventricular zone hand zygote ganglionic eminence embryo secondary oocyte More reference expression data BioGPS n/a
Gene ontology Molecular function DNA binding zinc ion binding metal ion binding nucleic acid binding DNA-binding transcription factor activity, RNA polymerase II-specific Cellular component intracellular anatomical structure nucleus Biological process regulation of transcription, DNA-templated transcription, DNA-templated regulation of transcription by RNA polymerase II Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 7738 193452 Ensembl ENSG00000096654 ENSMUSG00000006720 UniProt Q99676 Q7TSH9 RefSeq (mRNA) NM_007149 NM_001318891 NM_001318892 NM_001318893 NM_001347832 NM_183014 NM_001360886 RefSeq (protein) NP_001305820 NP_001305821 NP_001305822 NP_001334761 NP_009080 NP_898835 NP_001347815 Location (UCSC) Chr 6: 27.45 – 27.47 Mb Chr 13: 22.13 – 22.14 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

The National Center for Biotechnology Information (NCBI) Gene database entry^[5] for ZNF184 identifies conserved domains KRAB_A (Krüppel associated box) near the N-terminus and Zn-finger (Zinc finger) at the C-terminus of the translated protein. The former is associated with transcription repression^[7] and the latter with DNA binding (see Zinc finger).

Domains and Structure

The figure below is a reformatted and annotated conceptual translation display of ZNF184's Consensus CDS.^[8] CCDS displays exons in alternating black and blue font, with red indicating a residue coded across a splice boundary.

ZNF184 has 19 zinc finger motifs at the end of its final and longest exon. The figure shows regularity among the fingers in this protein, including the 2 columns of green-highlighted Cysteine residues and the 2 columns of blue-highlighted His residues which are the reason this type of zinc finger is called C2H2. Light grey highlighted columns (one with all F; one with mostly L, and F substitutions) are highly conserved hydrophobic residues within the zinc finger motif. The other light grey highlighted column (mostly K, with a similar R substitution) is an example of fairly strong conservation in the coil sections connecting adjacent fingers.

Near the N-terminus is a KRAB_A domain followed by a KRAB_B domain. KRAB_A has a shorter α-Helix followed by a longer α-Helix. The KRAB_A motif in a zinc finger protein is known to bind with a KAP-1 protein (aka TRIM28) to accomplish a transcription repressor function, however a gene so regulated by ZNF184 has yet to be identified. The length-11 finger helices are indicated, as well as the overlapping 7-residue section in each finger which binds targeted DNA (if the finger is functioning).

Reformatted Protein