Posizione | Batteria | Nazione | Atleti | Tempo | Note |
1 | 2 | Polonia | Kamila Ciba, Agata Forkasiewicz, Małgorzata Kołdej, Karolina Zagajewska | 44.10 | Q |
2 | 2 | Kazakistan | Rima Kashafutdinova, Viktoriya Zyabkina, Svetlana Golendova, Olga Safronova | 44.14 | Q |
3 | 1 | Svizzera | Ajla Del Ponte, Salomé Kora, Cornelia Halbheer, Selina von Jackowski | 44.23 | Q |
4 | 2 | Italia | Luminosa Bogliolo, Irene Siragusa, Anna Bongiorni, Ayomide Folorunso | 44.56 | q |
5 | 3 | Giappone | Sayaka Takeuchi, Mizuki Nakamura, Ichiko Iki, Miyu Maeyama | 44.71 | Q,  |
6 | 3 | Messico | Natali Brito, Dania Aguillón, Paola Morán, Iza Daniela Flores | 44.87 | Q,  |
7 | 2 | Stati Uniti | Justice Henderson, Samiyah Samuels, Tori Williams, Sierra Smith | 45.15 | q |
8 | 3 | Canada | Nyoka Maxwell, Jellisa Westney, Leah Walkeden, Sandra Latrace | 45.25 | |
9 | 2 | Taipei Cinese | Hsu Ya-ting, Ko Ching-ting, Syu Yong-jie, Hu Chia-chen | 45.26 |  |
10 | 1 | Australia | Jasmine Everett, Gabriella O'Grady, Elizabeth Hedding, Larissa Pasternatsky | 45.47 | Q,  |
11 | 1 | Thailandia | Parichat Charoensuk, Kanyarat Pakdee, Tassaporn Wannakit, Supawan Thipat | 45.60 | |
12 | 1 | Lettonia | Diāna Daktere, Anna Paula Auziņa, Ingūna Čeiko, Gunta Latiševa-Čudare | 46.16 |  |
13 | 2 | Danimarca | Annemarie Nissen, Mathilde Kramer, Astrid Glenner-Frandsen, Mette Graversgaard | 46.29 |  |
14 | 3 | Serbia | Ivana Petković, Nikoleta Šimić, Katarina Sirmić, Zorana Barjaktarović | 46.35 | |
15 | 3 | Uganda | Scovia Ayikoru, Mary Unyuthfua, Margaret Apolot, Leni Shida | 47.05 | |
16 | 3 | Botswana | Titose Chilume, Tsaone Sebele, Amantle Monwa, Ontiretse Molapisi | 48.40 | |
| 1 | Rep. Dominicana | Kiara Rodríguez, Estrella de Aza, Anabel Medina Ventura, Marileidy Paulino | DQ | R170.7 |
| 3 | Sudafrica | Justine Palframan, Maryke Brits, Lynique Prinsloo, Tamzin Thomas | DQ | R163.3a |
| 1 | Slovenia | | DNS | |