Acylphosphatase

In enzymology, an acylphosphatase (EC 3.6.1.7) is an enzyme that catalyzes the hydrolysis of the carboxyl-phosphate bond of acylphosphates, with acylphosphate and H₂O as the two substrates of this enzyme, and carboxylate and phosphate as its two products:^[3]

acylphosphatase
[1]
Identifiers
EC no.	3.6.1.7
CAS no.	9012-34-4
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Acylphosphatase
Structure of acylphosphatase.[2]
Identifiers
Symbol	Acylphosphatase
Pfam	PF00708
InterPro	IPR001792
PROSITE	PDOC00136
SCOP2	1aps / SCOPe / SUPFAM
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary PDB 2GV1​, 1APS​, 2FHM​, 1Y9O​, 1URR​, 1W2I​, 2ACY​, 2BJD​, 1V3Z​, 2HLT​, 2HLU​, 2BJE​, 3BR8​, 1ULR​

Function

This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing anhydrides. The systematic name of this enzyme class is acylphosphate phosphohydrolase. Other names in common use include acetylphosphatase, 1,3-diphosphoglycerate phosphatase, acetic phosphatase, Ho 1-3, and GP 1-3.

This enzyme participates in 3 metabolic pathways:

• glycolysis / gluconeogenesis
• pyruvate metabolism, and
• benzoate degradation via coa ligation.

Structural studies

Structures of this enzyme have been solved by both NMR and X-ray crystallography. See the links to PDB structures in the info boxes on the right for a current list of structures available in the PDB. The protein contains a beta sheet stacked on two alpha helices described by CATH as an Alpha-Beta Plait fold. The active site sits between sheet and helices and contains an arginine and an asparagine.^[4] Most structures are monomeric ^[5]

Isozymes

Humans express the following two acylphosphatase isozymes:

acylphosphatase 1, erythrocyte (common) type Identifiers Symbol ACYP1 NCBI gene 97 HGNC 179 OMIM 600875 RefSeq NM_001107 UniProt P07311 Other data EC number 3.6.1.7 Locus Chr. 14 q24.3 Search for Structures Swiss-model Domains InterPro

acylphosphatase 2, muscle type Identifiers Symbol ACYP2 NCBI gene 98 HGNC 180 OMIM 102595 RefSeq NM_138448 UniProt P14621 Other data EC number 3.6.1.7 Locus Chr. 2 p16.2 Search for Structures Swiss-model Domains InterPro