Estrone sulfotransferase

Estrone sulfotransferase (EST) (EC 2.8.2.4), also known as estrogen sulfotransferase, is an enzyme that catalyzes the transformation of an unconjugated estrogen like estrone into a sulfated estrogen like estrone sulfate. It is a steroid sulfotransferase and belongs to the family of transferases, to be specific, the sulfotransferases, which transfer sulfur-containing groups. This enzyme participates in androgen and estrogen metabolism and sulfur metabolism.

Estrone sulfotransferase
Identifiers
EC no.	2.8.2.4
CAS no.	9026-06-6
Alt. names	Estrogen sulfotransferase; EST
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Steroid sulfatase is an enzyme that catalyzes the reverse reaction, the transfer of a sulfate to an unconjugated estrogen.

Reaction

In enzymology, an EST is an enzyme that catalyzes the following chemical reaction:

3'-phosphoadenylyl sulfate + estrone ${\displaystyle \rightleftharpoons }$ adenosine 3',5'-bisphosphate + estrone 3-sulfate

Thus, the two substrates of this enzyme are 3'-phosphoadenylyl sulfate and estrone, whereas its two products are adenosine 3',5'-bisphosphate and estrone 3-sulfate.

The enzyme also catalyzes the same reaction for estradiol, with estradiol sulfate as the product.

Types

Two enzymes have been identified that together are thought to represent estrone sulfotransferase (EST):^[1][2]

• SULT1A1 (catalyzes the reactions estradiol to estradiol sulfate and, to a lesser extent than SULT1E1, estrone to estrone sulfate)
• SULT1E1 (catalyzes the reactions estrone to estrone sulfate and estradiol to estradiol sulfate)

Function

Distribution of STSTooltip steroid sulfatase and EST activities for interconversion of estradiol and estrone in adult human tissues.^[3]

Structure

As of late 2007, 5 structures have been solved for this class of enzymes, with PDB accession codes 1AQU, 1AQY, 1BO6, 1G3M, and 1HY3.

Names

The systematic name of this enzyme class is 3'-phosphoadenylyl-sulfate:estrone 3-sulfotransferase. Other names in common use include 3'-phosphoadenylyl sulfate-estrone 3-sulfotransferase, estrogen sulfotransferase, estrogen sulphotransferase, oestrogen sulphotransferase, and 3'-phosphoadenylylsulfate:oestrone sulfotransferase.

See also

• Steroidogenic enzyme
• Estrogen sulfotransferase