Steroid sulfotransferase

In enzymology, a steroid sulfotransferase (EC 2.8.2.15) is an enzyme that catalyzes the chemical reaction

3'-phosphoadenylyl sulfate + a phenolic steroid ${\displaystyle \rightleftharpoons }$ adenosine 3',5'-bisphosphate + steroid O-sulfate

steroid sulfotransferase
Identifiers
EC no.	2.8.2.15
CAS no.	9032-76-2
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Thus, the two substrates of this enzyme are 3'-phosphoadenylyl sulfate and phenolic steroid, whereas its two products are adenosine 3',5'-bisphosphate and steroid O-sulfate.

This enzyme belongs to the family of transferases, specifically the sulfotransferases, which transfer sulfur-containing groups. The systematic name of this enzyme class is 3'-phosphoadenylyl-sulfate:phenolic-steroid sulfotransferase. This enzyme is also called steroid alcohol sulfotransferase. This enzyme participates in steroid metabolism.

Genes

Of 62 sulfotransferase genes in the human genome, 16 represent cytoplasmic sulfotransferases, and of these 16 cytoplasmic sulfotransferases, five have been found to act as steroid sulfotransferases.^[1] These five sulfotransferase genes are SULT1A1, SULT1E1, and SULT2A1, as well as the two isoforms of SULT2B1, SULT2B1a and SULT2B1b.^[1] Their substrate specificity is as follows:^[1]

• SULT1A1: Estradiol (to estradiol sulfate)
• SULT1E1: DHEATooltip Dehydroepiandrosterone (to DHEA sulfate); Estrone (to estrone sulfate); Estradiol (to estradiol sulfate)
• SULT2A1: DHEA (to DHEA sulfate); Androsterone (to androsterone sulfate); Pregnenolone (to pregnenolone sulfate)
• SULT2B1a: Pregnenolone (to pregnenolone sulfate)
• SULT2B1b: Cholesterol (to cholesterol sulfate)

Traditionally, steroid sulfotransferases have been named according to their preferred substrate, for instance estrogen sulfotransferase (SULT1E1) and DHEA sulfotransferase (SULT2A1).^[1] However, cytosolic steroid sulfotransferases show broad substrate specificity, and SULT1E1 and SULT2A1 are not the only steroid sulfotransferases that sulfate estrogens and DHEA, respectively.^[1]

See also

• Estrogen sulfotransferase
• Estrone sulfotransferase
• Steroid sulfatase
• Steroidogenic enzyme