RAD17

Cell cycle checkpoint protein RAD17 is a protein that in humans is encoded by the RAD17 gene.^[5][6]

RAD17
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3A1J, 3G65, 3GGR
Identifiers
Aliases	RAD17, CCYC, HR24L, RAD17SP, RAD24, RAD17 checkpoint clamp loader component
External IDs	OMIM: 603139; MGI: 1333807; HomoloGene: 32117; GeneCards: RAD17; OMA:RAD17 - orthologs
Gene location (Human) Chr. Chromosome 5 (human)[1] Band 5q13.2 Start 69,369,293 bp[1] End 69,414,801 bp[1]
Gene location (Mouse) Chr. Chromosome 13 (mouse)[2] Band 13|13 D1 Start 100,753,672 bp[2] End 100,787,559 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in gonad testicle right testis left testis islet of Langerhans epithelium of colon endometrium tonsil Achilles tendon ganglionic eminence Top expressed in Gonadal ridge spermatid mandibular prominence atrioventricular valve seminiferous tubule maxillary prominence olfactory epithelium vas deferens atrium abdominal wall More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nucleotide binding protein binding ATP binding chromatin binding DNA clamp loader activity Cellular component telomere Rad17 RFC-like complex nucleus nucleoplasm nucleolus Biological process mitotic cell cycle checkpoint signaling DNA replication checkpoint signaling DNA replication DNA damage checkpoint signaling cell cycle regulation of phosphorylation negative regulation of DNA replication cellular response to DNA damage stimulus mitotic DNA replication checkpoint signaling DNA repair regulation of signal transduction by p53 class mediator Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5884 19356 Ensembl ENSG00000276618 ENSG00000152942 ENSMUSG00000021635 UniProt O75943 Q6NXW6 RefSeq (mRNA) NM_001278622 NM_002873 NM_133338 NM_133339 NM_133340 NM_133341 NM_133342 NM_133343 NM_133344 NM_001044371 NM_001283011 NM_011233 RefSeq (protein) NP_001265551 NP_002864 NP_579916 NP_579917 NP_579918 NP_579919 NP_579920 NP_579921 NP_579922 NP_001037836 NP_001269940 NP_035363 Location (UCSC) Chr 5: 69.37 – 69.41 Mb Chr 13: 100.75 – 100.79 Mb PubMed search [3] [4]
Wikidata
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Function

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported.^[7]

Meiosis

During meiosis in yeast and in mammals, RAD17 protein functions as a DNA damage sensor promoting DNA checkpoint control.^[8] In yeast, the RAD17 protein facilitates proper assembly of the meiotic crossover recombination complex containing the RAD51 protein, thus promoting efficient repair of meiotic DNA double-strand breaks.^[9] During male meiosis in maize (Zea mays), the ZmRAD17 gene is involved in repair of DNA double strand breaks, likely by promoting synaptonemal complex assembly.^[8]

Interactions

RAD17 has been shown to interact with:

• Ataxia telangiectasia and Rad3 related,^[10][11]
• Ataxia telangiectasia mutated,^[10][11]
• HUS1,^[12][13]
• NHP2L1,^[14]
• POLE,^[15]
• RAD1 homolog,^[10][12][13] and
• RAD9A.^{[12][13][16][17]}