Golgi reassembly-stacking protein 1

Golgi reassembly-stacking protein 1 (GORASP1) also known as Golgi reassembly-stacking protein of 65 kDa (GRASP65) is a protein that in humans is encoded by the GORASP1 gene.^[5]

GORASP1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4REY
Identifiers
Aliases	GORASP1, GOLPH5, GRASP65, P65, golgi reassembly stacking protein 1
External IDs	OMIM: 606867; MGI: 1921748; HomoloGene: 49916; GeneCards: GORASP1; OMA:GORASP1 - orthologs
Gene location (Human) Chr. Chromosome 3 (human)[1] Band 3p22.2 Start 39,095,222 bp[1] End 39,108,369 bp[1]
Gene location (Mouse) Chr. Chromosome 9 (mouse)[2] Band 9|9 F4 Start 119,754,323 bp[2] End 119,766,631 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right lobe of thyroid gland right uterine tube transverse colon minor salivary glands body of pancreas left lobe of thyroid gland body of stomach right lung rectum body of uterus Top expressed in yolk sac transitional epithelium of urinary bladder lacrimal gland otic vesicle saccule parotid gland right kidney proximal tubule interventricular septum muscle of thigh More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding metal ion binding Cellular component Golgi membrane Golgi apparatus endoplasmic reticulum-Golgi intermediate compartment membrane membrane Biological process protein transport endoplasmic reticulum to Golgi vesicle-mediated transport COPII vesicle coating protein N-linked glycosylation negative regulation of dendrite morphogenesis Golgi organization positive regulation of ubiquitin protein ligase activity establishment of protein localization to plasma membrane Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 64689 74498 Ensembl ENSG00000114745 ENSMUSG00000032513 UniProt Q9BQQ3 Q91X51 RefSeq (mRNA) NM_001278789 NM_001278790 NM_031899 NM_028976 RefSeq (protein) NP_001265718 NP_001265719 NP_114105 NP_114105.1 NP_083252 Location (UCSC) Chr 3: 39.1 – 39.11 Mb Chr 9: 119.75 – 119.77 Mb PubMed search [3] [4]
Wikidata
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Function

Microinjection of antibodies to GORASP1 prevents normal Golgi stack formation.^[6]

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The GORASP1 protein is a peripheral membrane protein anchored to the lipid bilayer through myristoylation of a glycine residue near the protein's amino terminus.^[7] It is involved in establishing the stacked structure of the Golgi apparatus and linking the stacks into larger ribbons in vertebrate cells.^[7] It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis.^[8][9] GORASP1 can form a complex with the Golgi matrix protein GM130, and this complex binds to the vesicle docking protein p115.^[7][a] Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined.^[5]

Structure

GORASP1 contains two PDZ domains in the amino-terminal GRASP domain (amino acid residues 2–210), that comprises approximately half of the protein. The GRASP region interacts with the Golgi matrix protein GM130 as well as an intrinsically disordered region in the C-terminus.^[7][10]

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  GRASP65 superhelix, topview^[7]
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  GRASP65 superhelix, side view^[7]
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Interactions

GORASP1 has been shown to interact with TGF alpha,^[11] TMED2^[11] and GOLGA2.^[11][12][13]

Notes

1. This is shown in the external link entitled "Molecular models of GRASP65/GM130/P115-mediated cis-cisternae membrane stacking and vesicle tethering."