TOP2A

DNA topoisomerase IIα is a human enzyme encoded by the TOP2A gene.

TOP2A
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1ZXM, 1ZXN, 4FM9, 4R1F
Identifiers
Aliases	TOP2A, TOP2, TP2A, topoisomerase (DNA) II alpha, DNA topoisomerase II alpha, TOPIIA, TOP2alpha
External IDs	OMIM: 126430; MGI: 98790; HomoloGene: 830; GeneCards: TOP2A; OMA:TOP2A - orthologs
Gene location (Human) Chr. Chromosome 17 (human)[1] Band 17q21.2 Start 40,388,525 bp[1] End 40,417,896 bp[1]
Gene location (Mouse) Chr. Chromosome 11 (mouse)[2] Band 11 D|11 62.91 cM Start 98,883,769 bp[2] End 98,915,015 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in ventricular zone ganglionic eminence secondary oocyte right testis left testis bone marrow trabecular bone gonad bone marrow cells testicle Top expressed in genital tubercle tail of embryo ventricular zone fetal liver hematopoietic progenitor cell maxillary prominence primitive streak mandibular prominence epiblast tibiofemoral joint somite More reference expression data BioGPS n/a
Gene ontology Molecular function DNA binding nucleotide binding ATP-dependent activity, acting on DNA protein homodimerization activity histone deacetylase binding chromatin binding isomerase activity metal ion binding DNA topoisomerase activity protein C-terminus binding ubiquitin binding protein binding protein heterodimerization activity enzyme binding ATP binding protein kinase C binding magnesium ion binding DNA binding, bending RNA binding DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity Cellular component cytoplasm DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex nucleoplasm nuclear chromosome nucleolus centriole condensed chromosome nucleus viral integration complex protein-containing complex ribonucleoprotein complex Biological process resolution of meiotic recombination intermediates apoptotic chromosome condensation embryonic cleavage rhythmic process chromosome segregation DNA metabolic process protein sumoylation cellular response to DNA damage stimulus DNA ligation chromosome condensation regulation of circadian rhythm hematopoietic progenitor cell differentiation positive regulation of apoptotic process mitotic DNA integrity checkpoint signaling positive regulation of viral genome replication positive regulation of single stranded viral RNA replication via double stranded DNA intermediate sister chromatid segregation positive regulation of transcription by RNA polymerase II DNA topological change negative regulation of DNA duplex unwinding female meiotic nuclear division Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 7153 21973 Ensembl ENSG00000131747 ENSMUSG00000020914 UniProt P11388 Q01320 RefSeq (mRNA) NM_001067 NM_011623 RefSeq (protein) NP_001058 NP_035753 Location (UCSC) Chr 17: 40.39 – 40.42 Mb Chr 11: 98.88 – 98.92 Mb PubMed search [3] [4]
Wikidata
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Topoisomerase IIα relieves topological DNA stress during transcription, condenses chromosomes, and separates chromatids. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several chemotherapy agents^[5] and a variety of mutations in this gene have been associated with the development of drug resistance.^{[citation needed]} Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.^[6]

Interactions

TOP2A has been shown to interact with SMURF2,^[7] HDAC1,^[8][9] CDC5L,^[10] Small ubiquitin-related modifier 1,^[11] P53,^[12] and TOPBP1.^[13]

In other species

In Drosophila Hadlaczky et al 1988 found DNA topoisomerase II α to correlate with cell proliferation - but β did not.^[14]

See also

• Topoisomerase II
• TOP2B - Topoisomerase II beta
• Gene duplication
• Ataxia-telangiectasia
• TOPBP1