VPS25

Vacuolar protein-sorting-associated protein 25 is a protein that in humans is encoded by the VPS25 gene.^[5][6]

VPS25
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2ZME, 3CUQ, 3HTU
Identifiers
Aliases	VPS25, DERP9, EAP20, FAP20, vacuolar protein sorting 25 homolog
External IDs	OMIM: 610907; MGI: 106354; HomoloGene: 6303; GeneCards: VPS25; OMA:VPS25 - orthologs
Gene location (Human) Chr. Chromosome 17 (human)[1] Band 17q21.2 Start 42,773,449 bp[1] End 42,779,599 bp[1]
Gene location (Mouse) Chr. Chromosome 11 (mouse)[2] Band 11 D|11 64.48 cM Start 101,144,533 bp[2] End 101,150,375 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in mucosa of transverse colon rectum islet of Langerhans skin of arm right uterine tube mucosa of ileum body of stomach smooth muscle tissue stromal cell of endometrium olfactory zone of nasal mucosa Top expressed in yolk sac tail of embryo genital tubercle right kidney lip embryo embryo granulocyte muscle of thigh blastocyst More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein homodimerization activity protein N-terminus binding structural molecule activity protein binding Cellular component cytoplasm cytosol endosome membrane nucleoplasm endosome membrane extracellular exosome nucleus ESCRT II complex Biological process regulation of transcription, DNA-templated multivesicular body assembly transcription, DNA-templated endosomal transport protein transport protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway macroautophagy negative regulation of epidermal growth factor-activated receptor activity transport multivesicular body sorting pathway Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 84313 28084 Ensembl ENSG00000131475 ENSMUSG00000078656 UniProt Q9BRG1 Q9CQ80 RefSeq (mRNA) NM_032353 NM_001284411 NM_001284412 NM_001284414 NM_026776 RefSeq (protein) NP_115729 NP_115729.1 NP_001271340 NP_001271341 NP_001271343 NP_081052 Location (UCSC) Chr 17: 42.77 – 42.78 Mb Chr 11: 101.14 – 101.15 Mb PubMed search [3] [4]
Wikidata
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ESCRT-II complex subunit
crystal structure of subunit vps25 of the endosomal trafficking complex escrt-ii
Identifiers
Symbol	ESCRT-II
Pfam	PF05871
InterPro	IPR008570
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

It is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT (ESCRT-I, -II, -III) complexes orchestrate efficient sorting of ubiquitinated transmembrane receptors to lysosomes via multivesicular bodies (MVBs).^[7] ESCRT-II recruits the transport machinery for protein sorting at MVB.^[8] In addition, the human ESCRT-II has been shown to form a complex with RNA polymerase II elongation factor ELL in order to exert transcriptional control activity. ESCRT-II transiently associates with the endosomal membrane and thereby initiates the formation of ESCRT-III, a membrane-associated protein complex that functions immediately downstream of ESCRT-II during sorting of MVB cargo. ESCRT-II in turn functions downstream of ESCRT-I, a protein complex that binds to ubiquitinated endosomal cargo.^[9]

ESCRT-II is a trilobal complex composed of two copies of vps25, one copy of vps22 and the C-terminal region of vps36. The crystal structure of vps25 revealed two winged-helix domains, the N-terminal domain of vps25 interacting with vps22 and vps36.^[10]