RFC4

Replication factor C subunit 4 is a protein that in humans is encoded by the RFC4 gene.^[5][6]

RFC4
Identifiers
Aliases	RFC4, A1, RFC37, replication factor C subunit 4
External IDs	OMIM: 102577; MGI: 2146571; HomoloGene: 6288; GeneCards: RFC4; OMA:RFC4 - orthologs
Gene location (Human) Chr. Chromosome 3 (human)[1] Band 3q27.3 Start 186,789,880 bp[1] End 186,807,058 bp[1]
Gene location (Mouse) Chr. Chromosome 16 (mouse)[2] Band 16|16 B1 Start 22,932,693 bp[2] End 22,946,487 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in ventricular zone ganglionic eminence gonad rectum testicle cerebellar hemisphere bone marrow right hemisphere of cerebellum mucosa of transverse colon appendix Top expressed in medial ganglionic eminence maxillary prominence mandibular prominence otic placode Gonadal ridge abdominal wall otic vesicle fetal liver hematopoietic progenitor cell endocardial cushion Paneth cell More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nucleotide binding DNA binding single-stranded DNA helicase activity protein binding enzyme binding ATP binding DNA clamp loader activity Cellular component DNA replication factor C complex Ctf18 RFC-like complex nucleus nucleoplasm Elg1 RFC-like complex Biological process nucleotide-excision repair, DNA gap filling error-free translesion synthesis DNA strand elongation involved in DNA replication error-prone translesion synthesis DNA replication positive regulation of DNA-directed DNA polymerase activity translesion synthesis transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision nucleotide-excision repair, DNA incision, 5'-to lesion DNA repair telomere maintenance via semi-conservative replication DNA-dependent DNA replication regulation of signal transduction by p53 class mediator Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5984 106344 Ensembl ENSG00000163918 ENSMUSG00000022881 UniProt P35249 Q99J62 RefSeq (mRNA) NM_181573 NM_002916 NM_145480 RefSeq (protein) NP_002907 NP_853551 NP_663455 Location (UCSC) Chr 3: 186.79 – 186.81 Mb Chr 16: 22.93 – 22.95 Mb PubMed search [3] [4]
Wikidata
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Function

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.^[6]

Radioresistance of esophageal squamous carcinoma cells is a critical factor leading to treatment failure and recurrence.^[7] RFC4 expression is significantly upregulated in esophageal squamous carcinoma cell tissues and cells, particurly in cases that are radioresistant.^[7] RFC4 plays a role in conferring radioresistance to esophageal squamous cell carcinoma by bolstering repair of DNA damage.^[7] Thus RFC4 is a promising therapeutic target for combating radioresistance in esophageal squamous cell carcinoma.^[7]

Interactions

RFC4 has been shown to interact with:

• BRD4,^[8]
• CHTF18,^[9]
• PCNA,^[10][11]
• RFC2,^[10][12]
• RFC3,^[13][14] and
• RFC5.^{[10][12][13][15]}