POLR2A

DNA-directed RNA polymerase II subunit RPB1, also known as RPB1, is an enzyme that is encoded by the POLR2A gene in humans.

POLR2A
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2GHQ, 2GHT, 2LTO, 3D9K, 3D9L, 3D9M, 3D9N, 3D9O, 3D9P, 4JXT, 5IY9, 5IYA, 5IYC, 5IYB, 5IY7, 5IY8, 5IYD, 5IY6
Identifiers
Aliases	POLR2A, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1, polymerase (RNA) II subunit A, RNA polymerase II subunit A, NEDHIB
External IDs	OMIM: 180660; MGI: 98086; HomoloGene: 721; GeneCards: POLR2A; OMA:POLR2A - orthologs
EC number	2.7.7.48
Gene location (Human) Chr. Chromosome 17 (human)[1] Band 17p13.1 Start 7,484,366 bp[1] End 7,514,616 bp[1]
Gene location (Mouse) Chr. Chromosome 11 (mouse)[2] Band 11 B3|11 42.86 cM Start 69,624,823 bp[2] End 69,649,463 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right testis left testis stromal cell of endometrium mucosa of esophagus skin of leg right adrenal cortex right uterine tube pituitary gland left adrenal gland skin of abdomen Top expressed in neural layer of retina tail of embryo genital tubercle epiblast ventricular zone internal carotid artery Ileal epithelium tracheobronchial tree external carotid artery perirhinal cortex More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transferase activity DNA binding nucleotidyltransferase activity RNA polymerase II activity metal ion binding RNA-directed 5'-3' RNA polymerase activity DNA-directed 5'-3' RNA polymerase activity protein binding ubiquitin protein ligase binding RNA binding protein C-terminus binding promoter-specific chromatin binding Cellular component Prp19 complex nucleolus RNA polymerase II, core complex nucleus nucleoplasm cytoplasm Biological process mRNA splicing, via spliceosome regulation of transcription, DNA-templated somatic stem cell population maintenance DNA-templated transcription, termination transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping transcription by RNA polymerase II transcription, RNA-templated positive regulation of RNA splicing positive regulation of viral transcription transcription-coupled nucleotide-excision repair transcription initiation from RNA polymerase II promoter snRNA transcription by RNA polymerase II fibroblast growth factor receptor signaling pathway transcription, DNA-templated RNA metabolic process regulation of gene silencing by miRNA Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5430 20020 Ensembl ENSG00000284832 ENSG00000181222 ENSMUSG00000005198 UniProt P24928 P08775 RefSeq (mRNA) NM_000937 NM_009089 NM_001291068 RefSeq (protein) NP_000928 NP_001277997 Location (UCSC) Chr 17: 7.48 – 7.51 Mb Chr 11: 69.62 – 69.65 Mb PubMed search [3] [4]
Wikidata
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Function

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA-binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.^[5]

Interactions

POLR2A has been shown to interact with:

• BRCA1,^[6][7][8][9]
• CREBBP,^[10]
• CTDP1,^[7]
• CDK8,^[10]
• GTF2B,^[7]
• GTF2F1,^[7][10]
• GTF2H4,^[7]
• MED21,^[7][10][11]
• MED26,^[12]
• PCAF,^[10]
• POLR2C,^[13]
• POLR2E,^[13]
• POLR2H,^[13]
• POLR2L,^[13]
• PQBP1,^[14]
• SMARCA2,^[10][15][16]
• SMARCA4^[15][16][17]
• SMARCB1,^[10][15][16]
• SMYD3,^[18]
• SND1,^[19]
• SUPT5H,^[20][21]
• TAF11,^[7]
• TBP,^[7]
• TCEA1,^[22][23][24]
• TCERG1,^[25] and
• ZNF74.^[26]